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flow cytometry data acquisition and analysis  (Johns Hopkins HealthCare)

 
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    Johns Hopkins HealthCare flow cytometry data acquisition and analysis
    ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow <t>cytometry</t> data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.
    Flow Cytometry Data Acquisition And Analysis, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+data+acquisition+and+analysis/flow+cytometry/pmc05767863-356-24-14
    Average 90 stars, based on 1 article reviews
    flow cytometry data acquisition and analysis - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells"

    Article Title: A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA117.000195

    ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow cytometry data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.
    Figure Legend Snippet: ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow cytometry data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.

    Techniques Used: Labeling, Immunofluorescence, Staining, Concentration Assay, Flow Cytometry

    Related Articles

    Flow Cytometry:

    Article Title: A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells
    Article Snippet: .. We thank Dr. Hao Zhang from the Flow Cytometry and Immunology Core Facility at Johns Hopkins Bloomberg School of Public Health for assistance in flow cytometry data acquisition and analysis. .. The Zeiss confocal microscope was supported through National Institutes of Health shared instrumentation Grant S10OD016374.



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    ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow <t>cytometry</t> data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.
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    Image Search Results


    ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow cytometry data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.

    Journal: The Journal of Biological Chemistry

    Article Title: A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells

    doi: 10.1074/jbc.RA117.000195

    Figure Lengend Snippet: ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow cytometry data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.

    Article Snippet: We thank Dr. Hao Zhang from the Flow Cytometry and Immunology Core Facility at Johns Hopkins Bloomberg School of Public Health for assistance in flow cytometry data acquisition and analysis.

    Techniques: Labeling, Immunofluorescence, Staining, Concentration Assay, Flow Cytometry